Biotin—(propionyl-CoA-carboxylase (ATP-hydrolyzing)) ligase

In enzymology, a biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase (EC 6.3.4.10) is an enzyme that catalyzes the chemical reaction

ATP + biotin + apo-propionyl-CoA:carbon-dioxide ligase (ADP-forming) AMP + diphosphate + propionyl-CoA:carbon-dioxide ligase (ADP-forming)

The 3 substrates of this enzyme are ATP, biotin, and [[apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]], whereas its 3 products are AMP, diphosphate, and propionyl-CoA:carbon-dioxide ligase (ADP-forming).

This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The systematic name of this enzyme class is biotin:apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] ligase (AMP-forming). Other names in common use include biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] synthetase, biotin-propionyl coenzyme A carboxylase synthetase, and propionyl coenzyme A holocarboxylase synthetase. This enzyme participates in biotin metabolism.

References

• Siegel L, Foote JL and Coon MJ (1965). "The enzymatic synthesis of propionyl coenzyme A holocarboxylase from d-biotinyl 5'-adenylate and the apocarboxylase". J. Biol. Chem. 240: 1025–1031. PMID 14284697.